Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

ARPC4 gene silencing inhibits T24 cell invasion and metastasis via a mechanism involving Arp2/3/cofilin-1 signaling pathway

Shunyi Pang¹, Zeqin Yao², Chao Wang³, Guoqiang Chen²

¹Department of Urology, Zhaoqing City Gaoyao District People's Hospital, Zhaoqing 526100; ²Department of Urology, The Central Hospital of Sanya, Sanya 572000; ³Department of Urology, The First People's Hospital of Jingmen, Jingmen 448000, China.

For correspondence:- Guoqiang Chen Email: frxdr1@163.com

Accepted: 28 September 2020 Published: 30 October 2020

Citation: Pang S, Yao Z, Wang C, Chen G. ARPC4 gene silencing inhibits T24 cell invasion and metastasis via a mechanism involving Arp2/3/cofilin-1 signaling pathway. Trop J Pharm Res 2020; 19(10):2073-2078 doi: 10.4314/tjpr.v19i10.8

© 2020 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: To study the influence of ARPC4 gene silencing on human urinary bladder cancer (T24) cell proliferation, invasiveness and migration, and the mechanism(s) involved.

Methods: Short interfering RNA (siRNA) ARPC4 silencing fragment was transfected into T24 cells. Transfection efficiency was measured with qRT-PCR. Cell proliferation, invasiveness and migratory potential were determined with CCK-8, Transwell invasion assay, and immunofluorescence assay, respectively. Protein expressions of ARPC4 and cofilin-1 were assayed using Western blotting.

Results: Short interfering RNA (siRNA) silencing of ARPC4 gene led to the downregulation of mRNA and protein expressions of ARPC4 (t = 14.898, p < 0.05; t = 7.686, p < 0.05). It also significantly downregulated cofilin-1 protein, while inhibiting proliferative capacity, invasiveness and pseudopodia-formation capacity of T24 cells (t = 8.042, p < 0.05).

Conclusion: The results obtained suggest that ARPC4 gene silencing inhibits T24 cell invasion and metastasis via a mechanism involving regulation of the Arp2/3/cofilin-1 signaling route. This provides new leads for gene therapy.

Keywords: ARPC4, Bladder carcinoma, Gene silencing, Invasiveness, Cell proliferation